VEGF Gene Polymorphism Among Diabetes Mellitus and Diabetic Retinopathy
VEGF Gene Polymorphism in Diabetes
DOI:
https://doi.org/10.54393/fbt.v2i01.19Keywords:
Gene Polymorphism, VEGF, Diabetes, Diabetic RetinopathyAbstract
Vascular endothelial growth factor (VEGF) is a major angiogenic factor and a prime regulator of endothelial cell proliferation. VEGF gene is located on chromosome 6 (6p21.3). Objectives: To determine whether deletion at -2549 position of promoter region of the VEGF gene exert influence on the development of diabetic retinopathy. Methods: Diseased and control subjects were selected for blood sampling. The blood samples (n=50) was taken from diabetic retinopathy patients and blood samples(n=50) was taken from type 2 diabetes mellitus patients as control group. After DNA extraction Polymerase Chain Reaction was performed to amplify the VEGF gene and sequencing was commercially done for molecular analysis of VEGF gene. Results: The molecular analysis confirmed that deletion at -2549 position of the promoter region of VEGF significantly greater in DR group. 2-11 % deletion was examined at -2549 position of promoter region of VEGF. The DD genotype was responsible for the development of DR. Conclusions: This study indicates that DD gene mutation and D allele is an autonomous hazard aspect for the advancement and progression of retinopathy in people with type 2 diabetes, additionally different issues for example diabetic age and family ancestry of diabetes assumes key function in the advancement of retinopathy in diabetic patients.
References
Alkwikbi HF, Abdelfattah NM, Abdelfattah HM. International Journal of Medicine in Developing Countries. 2017; 1(1): 33-37. doi: 10.24911/IJMDC.1.1.6
McCombe M, Lightman S, Eckland DJ, Hamilton AM, Lightman SL. Effect of a long-acting somatostatin analogue (BIM23014) on proliferative diabetic retinopathy: a pilot study. Eye. 1991 Sep; 5(5): 569-75. doi: 10.1038/eye.1991.99
Tong L, Vernon SA, Kiel W, Sung V, Orr GM. Association of macular involvement with proliferative retinopathy in type 2 diabetes. Diabetic Medicine. 2001 May; 18(5): 388-94. doi: 10.1046/j.1464-5491.2001.00483.x
Klein R, Knudtson MD, Lee KE, Gangnon R, Klein BE. The Wisconsin Epidemiologic Study of Diabetic Retinopathy XXIII: the twenty-five-year incidence of macular edema in persons with type 1 diabetes. Ophthalmology. 2009 Mar 1; 116(3): 497-503. doi: 10.1016/j.ophtha.2008.10.016
El-Asrar AM, Struyf S, Mohammad G, Gouwy M, Rytinx P, Siddiquei MM, Hernández C, Alam K, Mousa A, De Hertogh G, Opdenakker G. Osteoprotegerin is a new regulator of inflammation and angiogenesis in proliferative diabetic retinopathy. Investigative Ophthalmology & Visual Science. 2017 Jun 1; 58(7): 3189-201. doi: 10.1167/iovs.16-20993
Carmeliet P. Angiogenesis in health and disease. Nature medicine. 2003 Jun 1; 9(6): 653-60. doi: 10.1038/nm0603-653
Zepeda-Romero LC, Barrera-de-Leon JC, Camacho-Choza C, Bernal CG, Camarena-Garcia E, Diaz-Alatorre C, Gutierrez-Padilla JA, Gilbert C. Retinopathy of prematurity as a major cause of severe visual impairment and blindness in children in schools for the blind in Guadalajara city, Mexico. British Journal of Ophthalmology. 2011 Nov ; 95(11): 1502-5. doi: 10.1136/bjophthalmol-2011-300015
Yoshikawa N, Noda K, Shinoda H, Uchida A, Ozawa Y, Tsubota K, Mashima Y, Ishida S. Serum vascular adhesion protein-1 correlates with vascular endothelial growth factor in patients with type II diabetes. Journal of Diabetes and its Complications. 2013 Mar; 27(2): 162-6. doi: 10.1016/j.jdiacomp.2012.09.001
Kusuhara S, Fukushima Y, Ogura S, Inoue N, Uemura A. Pathophysiology of diabetic retinopathy: the old and the new. Diabetes & metabolism journal. 2018 Oct; 42(5): 364-76. doi: 10.4093/dmj.2018.0182
Clermont AC, Aiello LP, Mori F, Aiello LM, Bursell SE. Vascular endothelial growth factor and severity of nonproliferative diabetic retinopathy mediate retinal hemodynamics in vivo: a potential role for vascular endothelial growth factor in the progression of nonproliferative diabetic retinopathy. American journal of ophthalmology. 1997 Oct 1; 124(4): 433-46. doi: 10.1016/S0002-9394(14)70860-8
Hietala K, Forsblom C, Summanen P, Groop PH, FinnDiane Study Group. Heritability of proliferative diabetic retinopathy. Diabetes. 2008 Aug; 57(8): 2176-80. doi: 10.2337/db07-1495
Morello CM. Etiology and natural history of diabetic retinopathy: an overview. American Journal of Health-System Pharmacy. 2007 Sep; 64(17): S3-7. doi: 10.2146/ajhp070330
Szaflik JP, Wysocki T, Kowalski M, Majsterek I, Borucka AI, Blasiak J, Szaflik J. An association between vascular endothelial growth factor gene promoter polymorphisms and diabetic retinopathy. Graefe's Archive for Clinical and Experimental Ophthalmology. 2008 Jan; 246: 39-43. doi: 10.1007/s00417-007-0674-6
Buraczynska M, Ksiazek P, Baranowicz-Gaszczyk I, Jozwiak L. Association of the VEGF gene polymorphism with diabetic retinopathy in type 2 diabetes patients. Nephrology dialysis transplantation. 2007 Mar; 22(3): 827-32. doi: 10.1093/ndt/gfl641
Fouad H, Mona A, Abdel H, Amira A, Abdel A. Vascular endothelial growth factor (VEGF) gene insertion/deletion polymorphism and diabetic retinopathy in patients with type 2 diabetes. Journal of American Science. 2011; 7(3): 199-205.
Watson CJ, Webb NJ, Bottomley MJ, Brenchley PE. Identification of polymorphisms within the vascular endothelial growth factor (VEGF) gene: correlation with variation in VEGF protein production. Cytokine. 2000 Aug; 12(8): 1232-5. doi: 10.1006/cyto.2000.0692
Churchill AJ, Carter JG, Ramsden C, Turner SJ, Yeung A, Brenchley PE, et al. VEGF polymorphisms are associated with severity of diabetic retinopathy. Investigative ophthalmology & visual science. 2008 Aug; 49(8): 3611-6. doi: 10.1167/iovs.07-1383
Yang B, Cross DF, Ollerenshaw M, Millward BA, Demaine AG. Polymorphisms of the vascular endothelial growth factor and susceptibility to diabetic microvascular complications in patients with type 1 diabetes mellitus. Journal of Diabetes and its Complications. 2003 Jan; 17(1): 1-6. doi: 10.1016/S1056-8727(02)00181-2
Awata T, Kurihara S, Takata N, Neda T, Iizuka H, Ohkubo T, et al. Functional VEGF C-634G polymorphism is associated with development of diabetic macular edema and correlated with macular retinal thickness in type 2 diabetes. Biochemical and biophysical research communications. 2005 Aug; 333(3): 679-85. doi: 10.1016/j.bbrc.2005.05.167
Stevens A, Soden J, Brenchley PE, Ralph S, Ray DW. Haplotype analysis of the polymorphic human vascular endothelial growth factor gene promoter. Cancer research. 2003 Feb; 63(4): 812-6.
Kapahi R, Manjari M, Uppal MS, Singh NR, Sambyal V, Guleria K. Association of− 2549 insertion/deletion polymorphism of vascular endothelial growth factor with breast cancer in North Indian patients. Genetic testing and molecular biomarkers. 2013 Mar; 17(3): 242-8. doi: 10.1089/gtmb.2012.0222
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