Assessment of TNF-α Gene Expression in Type 2 Diabetes Patients with Nephropathy: Type 2 Diabetes Patients with Nephropathy

Saima Sharif; Samra Anees; Muhammad Roman; Shah Jahan

doi:10.54393/fbt.v2i02.17

Authors

Saima Sharif Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Samra Anees Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Muhammad Roman Department of Microbiology, University of Health Sciences, Lahore, Pakistan
Shah Jahan Department of Immunology, University of Health Sciences, Lahore, Pakistan

DOI:

https://doi.org/10.54393/fbt.v2i02.17

Keywords:

Type 2 Diabetes, TNF-α, Gene Expression

Abstract

Type 2 diabetes mellitus (T2DM) and its complications, including nephropathy, are a significant public health concern worldwide. Tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine that plays a crucial role in the development and progression of chronic inflammatory diseases, including T2DM and diabetic nephropathy. Objective: To evaluate of TNF-α gene expression in patients with T2DM and nephropathy compared to healthy individuals. Methods: The cross-sectional study conducted on 120 individuals divided into three groups: healthy individuals, type 2 diabetes mellitus (T2DM) patients without diabetic nephropathy, and T2DM patients with nephropathy. RNA was extracted and TNF-α gene expression was evaluated using PCR and statistical analysis was done using SPSS software. Results: The results showed almost 4.2-fold induced expression of TNF-α in T2DM patients without nephropathy compared to the normal group. Conclusion: The study reports that in diabetic nephropathy patients, Gene expression of gene TNF-a shows increases in cases when compared with healthy subjects

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