Exploring the Relationship between TNF-α Gene Expression in Non Diabetic Nephropathy Type 2 Diabetes Patients: Relationship between TNF-α Gene Expression and Non Diabetic Nephropathy

Muhammad Roman; Samra Anees; Saima Sharif; Shah Jahan

doi:10.54393/fbt.v2i01.16

Authors

Muhammad Roman Department of Microbiology, University of Health Sciences, Lahore, Pakistan
Samra Anees Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Saima Sharif Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Shah Jahan Department of Immunology, University of Health Sciences, Lahore, Pakistan

DOI:

https://doi.org/10.54393/fbt.v2i01.16

Keywords:

TNF-α, Non Diabetic Nephropathy, Type 2 Diabetes Mellitus

Abstract

Non-diabetic nephropathy (NDN) is a common complication of type 2 diabetes, leading to kidney damage and impaired kidney function. TNF-α (tumor necrosis factor-alpha) is a pro-inflammatory cytokine that has been implicated in the development of NDN. Objective: To evaluate the gene expression of TNF-α in patients with type 2 diabetes mellitus (T2DM) without nephropathy to gain insight into the potential role of TNF-α in the pathogenesis of diabetic nephropathy (DN). Methods: Total of 80 subjects were tested, split into two groups, including healthy patients, T2DM patients without nephropathy, and T2DM patients with nephropathy. RNA was extracted from blood samples, and RT-PCR was used to observe the impact of T2DM without nephropathy on the expression of the TNF-α gene using gene-specific primers and SYBR Green mix. Results: The results showed almost 4.4-fold induced expression of TNF-α in T2DM patients without nephropathy compared to the normal group. Conclusion: The findings may have implications for the development of new therapies and biomarkers for DN, and for a good interpretation of the complex pathophysiology of T2DM. The exact role of TNF-α in the pathogenesis of DN in humans is not fully understood, and further investigation is needed.

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