Evaluation of Gene Expression of TNF-α in Healthy Subjects: Gene Expression of TNF-α

Samra Anees; Saima Sharif; Muhammad Roman; Shah Jahan

doi:10.54393/fbt.v1i01.15

Authors

Samra Anees Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Saima Sharif Department of Zoology, Lahore College for Women University, Lahore, Pakistan
Muhammad Roman Department of Microbiology, University of Health Sciences, Lahore, Pakistan
Shah Jahan Department of Immunology, University of Health Sciences, Lahore, Pakistan

DOI:

https://doi.org/10.54393/fbt.v1i01.15

Keywords:

Healthy Subjects, TNF-α, Gene Expression

Abstract

Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in the immune response, inflammation, and apoptosis. Dysregulation of TNF-α expression has been associated with various diseases, including autoimmune disorders, cancer, and chronic inflammatory conditions. Understanding the regulation of TNF-α expression in healthy individuals can help identify potential therapeutic targets for these diseases. Objective: To evaluate of gene expression of TNF-α in healthy subjects. Methods: The cross-sectional study conducted on 40 individuals on healthy individuals. RNA was extracted and TNF-α gene expression was evaluated using PCR and statistical analysis was done using SPSS software. Results: The evaluation of TNF-α gene expression in healthy individuals has also led to the identification of potential biomarkers of disease and new therapeutic targets. Conclusion: In conclusion, the evaluation of TNF-α gene expression in healthy individuals is an important tool for identifying potential biomarkers of disease and understanding the physiological role of this cytokine.

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