Development and Validation of Loop-Mediated Isothermal Amplification (Lamp) Field Assay for the Detection of Brucella abortus

Detection of Brucella abortus

Authors

  • Muhammad Jamil Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Shehzad Ali Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Ali Hussain Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Yassar Abbas Department of Animal Sciences, University of Veterinary and Animal Sciences, Jhang, Pakistan
  • Muhammad Ahsan Raza Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Andleeb Aslam Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Ali Hasan Institute of Zoology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.54393/fbt.v4i02.119

Keywords:

Brucella abortus, BSCP31 Gene, Serum Screening, Rose Bengal Plate Test

Abstract

Currently, a number of techniques are available for detection of Brucella abortus (B. abortus) but these techniques are costly and specialized equipment are needed. Therefore, the development of a rapid, accurate, sensitive, and cost effective technique for identification of Brucella species is of high priority. Objective: The current research study was designed to detect Brucella species more rapidly. The current study area was conducted in district Lodhran, Punjab, Pakistan. Methods: A total 100 blood samples (50 cattle and 50 buffaloes) were collected. Serum samples were screened against B. abortus antibodies using Rose Bengal plate test (RBPT). The specific gene was designed by using NCBI website and whole genome of Brucella species. The primers were designed from Gene accession number 20404. Following primers were designed F3, B3, FIP, BIP, LF, LB, B4, and B5. The LAMP technique for BSCP31 gene was developed by using many concentrations of components and conditions. Results: The development and validation of LAMP assay for detection of B. abortus from bovine blood in the present study proved helpful in early detection of said pathogen in animal and humans. Conclusions: This study will be helpful in prevention and control of animal and human brucellosis in Pakistan.

References

Godfroid J, Cloeckaert A, Liautard JP, Kohler S, Fretin D, Walravens K et al. From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Veterinary Research. 2005; 36(3): 313-26. doi: 10.1051/vetres:2005003.

Pal M. Zoonoses. [2nd Edition]. India: Satyam Publishers; 2007: 98-99.

Priyantha MA. Identification of biovars of Brucella abortus in aborted cattle and buffaloes herd in Sri Lanka. Veterinary World. 2011 Dec; 4(12): 542. doi: 10.5455/vetworld.2011.542-545.

Wyatt HV. How Themistocles Zammit found Malta fever (brucellosis) to be transmitted by the milk of goats. Journal of the Royal Society of Medicine. 2005 Oct; 98(10): 451-4. doi: 10.1258/jrsm.98.10.451.

Nene V, Kole C, editors. Genome mapping and genomics in animal-associated microbes. Springer Science & Business Media; 2008 Nov. doi: 10.1007/978-3-540-74042-1.

Xavier MN, Costa ÉA, Paixão TA, Santos RL. The genus Brucella and clinical manifestations of brucellosis. Ciência Rural. 2009; 39: 2252-60. doi: 10.1590/S0103-84782009005000167.

De Jong MF and Tsolis RM. Brucellosis and type IV secretion. Future Microbiology. 2012 Jan; 7(1): 47-58. doi: 10.2217/fmb.11.136.

Hadush A and Pal M. Brucellosis-An infectious re-emerging bacterial zoonosis of global importance. International Journal of Livestock Research. 2013 Jan; 3(1): 28-34. doi: 10.5455/ijlr.20130305064802.

Cutler SJ, Whatmore AM, Commander NJ. Brucellosis-new aspects of an old disease. Journal of Applied Microbiology. 2005 Jun; 98(6): 1270-81. doi: 10.1111/j.1365-2672.2005.02622.x.

Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos EV. The new global map of human brucellosis. The Lancet Infectious Diseases. 2006 Feb; 6(2): 91-9. doi: 10.1016/S1473-3099(06)70382-6.

Qureshi KA, Parvez A, Fahmy NA, Abdel Hady BH, Kumar S, Ganguly A et al. Brucellosis: epidemiology, pathogenesis, diagnosis and treatment-a comprehensive review. Annals of Medicine. 2023 Dec; 55(2): 2295398. doi: 10.1080/07853890.2023.2295398.

Leyla G, Kadri G, Ümran OK. Comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. Veterinary Microbiology. 2003 May; 93(1): 53-61. doi: 10.1016/S0378-1135(02)00442-X.

Ilhan Z, Aksakal A, Ekin IH, Gülhan T, Solmaz H, Erdenlig S. Comparison of culture and PCR for the detection of Brucella melitensis in blood and lymphoid tissues of serologically positive and negative slaughtered sheep. Letters in Applied Microbiology. 2008 Mar; 46(3): 301-6. doi: 10.1111/j.1472-765X.2007.02309.x.

Marianelli C, Martucciello A, Tarantino M, Vecchio R, Iovane G, Galiero G. Evaluation of molecular methods for the detection of Brucella species in water buffalo milk. Journal of Dairy Science. 2008 Oct; 91(10): 3779-86. doi: 10.3168/jds.2008-1233.

Pérez-Sancho M, García-Seco T, Arrogante L, García N, Martinez I, Diez-Guerrier A et al. Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples. Research in Veterinary Science. 2013 Oct; 95(2): 489-94. doi: 10.1016/j.rvsc.2013.05.002.

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Research. 2000 Jun; 28(12): e63-. doi: 10.1093/nar/28.12.e63.

Dhama K, Karthik K, Chakraborty S, Tiwari R, Kapoor S, Kumar A et al. Loop-mediated isothermal amplification of DNA (LAMP): a new diagnostic tool lights the world of diagnosis of animal and human pathogens: a review. Pakistan journal of biological sciences: Pakistan Journal of Biological Sciences. 2014 Jan; 17(2): 151-66. doi: 10.3923/pjbs.2014.151.166.

Sousa AK, Guimarães BR, Beserra PA, Bezerra DC, Melo FD, Santos HP et al. Brucelose bovina em frigoríficos com Serviços de Inspeção Federal e Municipal no estado do Maranhão. Arquivos do Instituto Biológico. 2019 May; 86: e0832017. doi: 10.1590/1808-1657000832017.

Junior DG, Lima AM, Moraes GP. Diagnosis of bovine brucellosis in bulls by seroagglutination and seminal plasma agglutination tests. Semina: Ciências Agrárias. 2015 Oct; 36(5): 3203-9. doi: 10.5433/1679-0359.2015v36n5p3203.

Sambrook J and Russell DW. Fragmentation of DNA by sonication. Cold spring harbor protocols. 2006 Sep; 2006(4): pdb-rot4538. doi: 10.1101/pdb.prot4538.

Boschiroli ML, Foulongne V, O'Callaghan D. Brucellosis: a worldwide zoonosis. Current opinion in microbiology. 2001 Feb; 4(1): 58-64. doi: 10.1016/S1369-5274(00)00165-X.

Tachibana M, Watanabe K, Kim S, Omata Y, Murata K, Hammond T et al. Antibodies to Brucella spp. in Pacific bottlenose dolphins from the Solomon Islands. Journal of Wildlife Diseases. 2006 Apr; 42(2): 412-4. doi: 10.7589/0090-3558-42.2.412.

Grammont-Cupillard M, Berthet-Badetti L, Dellamonica P. Brucellosis from sniffing bacteriological cultures. The Lancet. 1996 Dec; 348(9043): 1733-4. doi: 10.1016/S0140-6736(05)65855-2.

Fiori PL, Mastrandrea S, Rappelli P, Cappuccinelli P. Brucella abortus infection acquired in microbiology laboratories. Journal of Clinical Microbiology. 2000 May; 38(5): 2005-6. doi: 10.1128/JCM.38.5.2005-2006.2000.

Noviello S, Gallo R, Kelly M, Limberger RJ, DeAngelis K, Cain L et al. Laboratory-acquired brucellosis. Emerging Infectious Diseases. 2004 Oct; 10(10): 1848. doi: 10.3201/eid1010.040076.

Young EJ. An overview of human brucellosis. Clinical Infectious Diseases. 1995 Aug; 21(2): 283-9. doi; 10.1093/clinids/21.2.283.

Muñoz PM, Marín CM, Monreal D, Gonzalez D, Garin-Bastuji B, Diaz R et al. Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O: 9. Clinical and Vaccine Immunology. 2005 Jan; 12(1): 141-51. doi: 10.1128/CDLI.12.1.141-151.2005.

Klietmann WF and Ruoff KL. Bioterrorism: implications for the clinical microbiologist. Clinical Microbiology Reviews. 2001 Apr; 14(2): 364-81. doi: 10.1128/CMR.14.2.364-381.2001.

Batchelor BI, Brindle RJ, Gilks GF, Selkon JB. Biochemical mis-identification of Brucella melitensis and subsequent laboratory-acquired infections. Journal of Hospital Infection. 1992 Oct; 22(2): 159-62. doi: 10.1016/0195-6701(92)90100-Z.

Kortepeter MG and Parker GW. Potential biological weapons threats. Emerging infectious diseases. 1999 Jul; 5(4): 523. doi: 10.3201/eid0504.990411.

DelVecchio VG, Kapatral V, Redkar RJ, Patra G, Mujer C, Los T et al. The genome sequence of the facultative intracellular pathogen Brucella melitensis. Proceedings of the National Academy of Sciences. 2002 Jan; 99(1): 443-8. doi: 10.1073/pnas.221575398.

Queipo-Ortuño MI, Colmenero JD, Reguera JM, García-Ordoñez MA, Pachón ME, Gonzalez M et al. Rapid diagnosis of human brucellosis by SYBR Green I-based real-time PCR assay and melting curve analysis in serum samples. Clinical Microbiology and Infection. 2005 Sep; 11(9): 713-8. doi: 10.1111/j.1469-0691.2005.01202.x.

Parida M, Sannarangaiah S, Dash PK, Rao PV, Morita K. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Reviews in Medical Virology. 2008 Nov; 18(6): 407. doi: 10.1002/rmv.593.

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Published

2024-06-30
CITATION
DOI: 10.54393/fbt.v4i02.119
Published: 2024-06-30

How to Cite

Jamil, M., Ali, S., Hussain, A., Abbas, Y., Raza, M. A., Aslam, A., & Hasan, A. (2024). Development and Validation of Loop-Mediated Isothermal Amplification (Lamp) Field Assay for the Detection of Brucella abortus: Detection of Brucella abortus. Futuristic Biotechnology, 4(02), 19–24. https://doi.org/10.54393/fbt.v4i02.119

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