Determining Acid-Bile Optimization and Correlation between Optical Density and the Colony Forming Units of Lactobacilli Species: Optimizing Acid-Bile Tolerance of Lactobacilli

Shamsa Jabeen; Ali Hasan; Muhammad Rizwan; Hina Javed; Muhammad Ahsan Raza; Shehzad Ahmad; Ali Hussain

doi:10.54393/fbt.v4i01.101

Authors

Shamsa Jabeen Institute of Zoology, University of the Punjab, Lahore, Pakistan
Ali Hasan Honeybee Research Garden, Institute of Zoology, University of the Punjab, Lahore, Pakistan
Muhammad Rizwan Department of Botany, University of Veterinary and Animal Sciences, Lahore, Pakistan
Hina Javed Teratology Laboratory, Institute of Zoology, University of the Punjab, Lahore, Pakistan
Muhammad Ahsan Raza Endocrinology Laboratory, Institute of Zoology, University of the Punjab, Lahore, Pakistan
Shehzad Ahmad Applied and Environmental Microbiology Laboratory, Institute of Zoology, University of the Punjab, Lahore, Pakistan
Ali Hussain Applied and Environmental Microbiology Laboratory, Institute of Zoology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.54393/fbt.v4i01.101

Keywords:

Probiotics, Lactobacilli, Colony Forming Unit, Optical Density

Abstract

Different research practices are being done with time to check the fruitful aspects of probiotics administration in food. In this regard, a specific number of probiotics is mixed in the food and presented to the experimental animals in laboratory to the fruitful impacts of probiotics on host. Objective: To design the standard curve between optical density and the number of colony-forming units. Methods: Lactobacilli species were grown in broth and their colony forming units (CFU) were calculated at different dilutions. In addition to this, relationship between optical density and number of colony forming units and acid-bile tolerance of these Lactobacilli species were also found out. Results: It was observed that L. paracasii, L. delbrueckii, L. rhamnosus, and L. brevis showed optimum rates with the Mean±SEM values of 2.56±0.04 on pH 7, 2.51±0.04 on pH 7.5, 2.46±0.04 on pH 7.5 and 2.54±0.02 on pH 7 respectively (p <0.0001). Similarly, all these isolates (L. paracasii, L. delbrueckii, L. rhamnosus, and L. brevis) showed maximum growth rates on bile concentration of 0.1, and their Mean±SEM values of optical densities were 1.83±0.10, 2.36±0.25, 2.50±0.31, 1.58±0.10 respectively (p <0.0001). Means were compared by ANOVA employing SPSS 20.0. Conclusions: The study provided insights in conducting different research practices on probiotics in future.

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