Exploring the Confluence of In-Ovo Mineral Supplementation and Hatching Attributes in Broiler Chick Progeny: A Symphony of Nutritional Enrichment and Broiler Chicks Development: In-Ovo Mineral Supplementation and Hatching

Imran Ahmed; Nasir Rajput; Imdad Hussain Laghari; Rameez Raja Kaleri; Shazia Shamas; Zulfiqar Ahmed; Farooque Ahmed Khushk; Nasir Mukhtar

doi:10.54393/fbt.v3i02.64

Authors

Imran Ahmed Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam, Pakistan
Nasir Rajput Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam, Pakistan
Imdad Hussain Laghari Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam, Pakistan
Rameez Raja Kaleri Deputy Director Poultry Production Tando Allahyar, Livestock and Fisheries Department Government of Sindh.
Shazia Shamas Department of Zoology, Rawalpindi Women University, Rawalpindi, Pakistan
Zulfiqar Ahmed Department of Livestock and Poultry Production, Faculty of Veterinary and Animal Sciences, University of Poonch Rawalakot, Azad Jammu and Kashmir, Pakistan
Farooque Ahmed Khushk Department of Poultry Husbandry, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam, Pakistan
Nasir Mukhtar Department of Livestock Production and Management, Faculty of Veterinary and Animal Sciences, Pir Mehar Ali Shah Arid Agriculture University, Rawalpindi, Pakistan

DOI:

https://doi.org/10.54393/fbt.v3i02.64

Keywords:

In-ovo minerals feeding, Hatchability, Hatch window, Chick weight, Hatchling length, Chick quality

Abstract

Embryonic nutrition is a key factor that influences broiler progeny performance with long-lasting nutritional adequacies or insufficiencies in the developmental phase. Objective: To explores the effects of in-ovo mineral supplementation or day in-ovo feeding on the hatching attributes and chick quality in broiler breeders. Methods: About 1400 hatching eggs of broiler breeders were selected, categorized into seven experimental groups: one control group, three macro mineral (P, Mg, and Ca) groups, and three micro-mineral (Zn, Mn, and Cu) groups. During the 12^th and 18^th day of incubation, eggs received in-ovo injections of their respective mineral (5% of the total mineral content in a 50g egg) solutions, prepared by dissolving the corresponding salts in deionized water. Post-injection, eggs were placed in the setter, and upon completion of incubation, hatching trays were removed to evaluate hatchability performance and chick quality parameters for each group. Results: The results showed that Zn, Ca, and Mg-supplemented eggs demonstrated the highest (P<0.05) hatchability rates for settable and fertile eggs at day 12 of in-ovo feeding. The chick weight, hatch window, and hatchling size were significantly influenced (P<0.05) by in-ovo feeding and the day of in-ovo feeding. However, no effect of in-ovo mineral feeding and day of in ovo feeding was observed on chick quality (chick grades, navel area, retracted yolk, and membrane score) and post-hatch chick activities (activity, down-appearance, vitality, eye score, and gait score). Conclusions: In conclusion, Zn, Ca, and Mg in ovo feeding improve the hatchability traits when administered at day 12 of incubation.

References

Yair R, Shahar R, Uni Z. Prenatal nutritional manipulation by in ovo enrichment influences bone structure, composition, and mechanical properties. Journal of Animal Science. 2013 Jun; 91(6): 2784-93. doi: 10.2527/jas.2012-5548.

Oviedo-Rondon EO, Leandro NM, Ali R, Koci M, Moraes V, Brake J. Broiler breeder feeding programs and trace minerals on maternal antibody transfer and broiler humoral immune response. Journal of Applied Poultry Research. 2013 Oct; 22(3): 499-510. doi: 10.3382/japr.2012-00708.

Das R, Mishra P, Jha R. In ovo feeding as a tool for improving performance and gut health of poultry: a review. Frontiers in Veterinary Science. 2021 Nov 11;8:754246. doi: 10.3389/fvets.2021.754246.

Awachat Vb, Elangovan Av, Sogunle Om, David Cg, Ghosh J, Gowda Sn, et al., Influence of in ovo and pre-starter zinc and copper supplementation on growth performance and gastrointestinal tract development of broiler chickens. Acta Agriculturae Slovenica. 2020 Jun; 115(2): 237-45. doi: 10.14720/aas.2020.115.2.562.

Oliveira TF, Bertechini AG, Bricka RM, Hester PY, Kim EJ, Gerard PD, et al., Effects of in ovo injection of organic trace minerals and post-hatch holding time on broiler performance and bone characteristics. Poultry Science. 2015 Nov; 94(11): 2677-85. doi: 10.3382/ps/pev249.

Uni Z and Ferket PR, inventors; Yissum Research Development Co of Hebrew University of Jerusalem, North Carolina State University, assignee. Enhancement of development of oviparous species by in ovo feeding. United States patent. 2003 Jul. US 6,592,878.

Uni Z and Ferket PR, inventors; Yissum Research Development Co of Hebrew University of Jerusalem, North Carolina State University, assignee. Enhancement of development of oviparous species by in ovo feeding of enteric modulators. 2014 May. United States patent US 8,734,837.

Yair R and Uni Z. Content and uptake of minerals in the yolk of broiler embryos during incubation and effect of nutrient enrichment. Poultry Science. 2011 Jul 1;90(7):1523-31. doi: 10.3382/ps.2010-01283.

Hassan HA, Arafat AR, Farroh KY, Bahnas MS, El-Wardany I, Elnesr SS. Effect of in ovo copper injection on body weight, immune response, blood biochemistry and carcass traits of broiler chicks at 35 days of age. Animal Biotechnology. 2022 Nov; 33(6): 1134-41. doi: 10.1080/10495398.2021.1874964.

Hassan HA, Arafat AR, Farroh KY, Bahnas MS, El-Wardany I, Elnesr SS. Histological alterations of small intestine and growth performance of broiler chicks after in ovo copper injection at 10 days of embryogenesis period. Animal Biotechnology. 2023 Jun; 34(3): 585-92. doi: 10.1080/10495398.2021.1985509.

Kim HJ and Kang HK. Effects of in ovo injection of zinc or diet supplementation of zinc on performance, serum biochemical profiles, and meat quality in broilers. Animals. 2022 Mar; 12(5): 630. doi: 10.3390/ani12050630.

Sogunle OM, Elangovan AV, David CG, Ghosh J, Awachat VB. Response of broiler chicken to in ovo administration of inorganic salts of zinc, selenium and copper or their combination. Slovak Journal of Animal Science. 2018 Mar; 51(1): 8-19.

Jose N, Elangovan AV, Awachat VB, Shet D, Ghosh J, David CG. Response of in ovo administration of zinc on egg hatchability and immune response of commercial broiler chicken. Journal of Animal Physiology and Animal Nutrition. 2018 Apr; 102(2): 591-5. doi: 10.1111/jpn.12777.

Joshua PP, Valli C, Balakrishnan V. Effect of in ovo supplementation of nano forms of zinc, copper, and selenium on post-hatch performance of broiler chicken. Veterinary World. 2016 Mar; 9(3): 287-94. doi: 10.14202/vetworld.2016.287-294.

Tona K, Bruggeman V, Onagbesan O, Bamelis F, Gbeassor M, Mertens K, et al., Day-old chick quality: Relationship to hatching egg quality, adequate incubation practice and prediction of broiler performance. Avian and Poultry Biology Reviews. 2005 Jan; 16(2): 109-19. doi: 10.3184/147020605783438787.

Khalil NM, do Nascimento TC, Casa DM, Dalmolin LF, de Mattos AC, Hoss I, et al., Pharmacokinetics of curcumin-loaded PLGA and PLGA–PEG blend nanoparticles after oral administration in rats. Colloids and Surfaces B: Biointerfaces. 2013 Jan; 101(1): 353-60. doi: 10.1016/j.colsurfb.2012.06.024

Akbari Moghaddam Kakhki, R., Z. Shahneh, A., Shivazad, M., & Sadeghi, G. In ovo feeding of zinc on hatchability and subsequent growth performance of Japanese quail. Revista Brasileira de Ciência Avícola. 2015 Feb; 17(2): 177-184.

Torres CA and Korver DR. Influences of trace mineral nutrition and maternal flock age on broiler embryo bone development. Poultry Science. 2018 Aug; 97(8): 2996-3003. doi: 10.3382/ps/pey136.

Zhang, J., Wang, J., Zhang, Y., Xu, Y., Lu, L., & Luo, X. Effects of in ovo feeding of calcium on hatchability, bone development, and mineral retention in broiler chicks. Poultry Science. 2017 Mar; 96(3): 640-648.

Nowaczewski S, Kontecka H, Krystianiak S. Effect of in ovo injection of vitamin C during incubation on hatchability of chickens and ducks. Folia Biologica (Kraków). 2012 Jan; 60(1-2): 93-7. doi; 10.3409/fb60_1-2.93-97.

El-Damrawy, S. Z., El-Sayed, M. S., & Abdoon, S. A. The effect of in ovo injection of zinc oxide nanoparticles on hatchability, chick quality, and productivity of broiler chickens. Veterinary World. 2019 Mar; 12(3): 414-420.

Joshua, P. E., Omede, A. A., Musa, U. Effects of in ovo feeding of zinc nanoparticles on hatchability, growth and survival of broiler chickens. Animal Production Science. 2016 Aug; 56(8): 1473-1477.

Sun X, Lu L, Liao X, Zhang L, Lin X, Luo X, et al., Effect of in ovo zinc injection on the embryonic development and epigenetics-related indices of zinc-deprived broiler breeder eggs. Biological trace element research. 2018 Oct; 185(10): 456-64. doi: 10.1007/s12011-018-1260-y.

Amen, S. A., El-Daraji, H. R. (). The effect of zinc supplementation on the productive performance and hatching traits of laying hens. International Journal of Poultry Science. 2011 Apr; 10(4): 288-294. doi: 10.3923/ijps.2011.288.294.

Rossi P, Rutz F, Anciuti MA, Rech JL, Zauk NH. Influence of graded levels of organic zinc on growth performance and carcass traits of broilers. Journal of Applied Poultry Research. 2007 Jul; 16(2): 219-25. doi: 10.1093/japr/16.2.219.

Yenice E, Mızrak C, Gültekin M, Atik Z, Tunca M. Effects of organic and inorganic forms of manganese, zinc, copper, and chromium on bioavailability of these minerals and calcium in late-phase laying hens. Biological trace element research. 2015 Oct; 167: 300-7. doi: 10.1007/s12011-015-0313-8.

Ola Awais Hamza, A., Yousaf, M. S., Khan, S., Khan, R. U. In ovo zinc oxide nanoparticles and zinc sulphate injection on hatchability, chick weight, and organ development of broiler chicken. International Journal of Nanomedicine. 2022; 17, 1919-1926.

Sahr WB, Odutayo OJ, Sogunle OM, Ayo-Ajasa OY, Fafiolu AO, Fatunmbi FA. Effects of in ovo injection of inorganic salts of Zn, Cu and Mn on hatching traits and post-hatch performance of broiler chickens in the tropics. Nigerian Journal of Animal Science. 2020 Jul; 22(1): 113-25.

Ohta Y and Kidd MT. Optimum site for in ovo amino acid injection in broiler breeder eggs. Poultry Science. 2001 Oct; 80(10): 1425-9. doi: 10.1093/ps/80.10.1425.

van der Eijk JA, Bakker J, Güz BC, van Krimpen MM, Molenaar R, van den Brand H, et al., Providing organic macro minerals and an elevated platform improved tibia characteristics, and increased locomotion and performance of fast-and slower-growing broilers. Poultry Science. 2022 Aug; 101(8): 101973. doi: 10.1016/j.psj.2022.101973.

Güz BC, de Jong IC, Bol UE, Kemp B, van Krimpen M, Molenaar R, et al., Effects of organic macro and trace minerals in fast and slower growing broiler breeders’ diet on offspring growth performance and tibia characteristics. Poultry Science. 2022 Mar; 101(3): 101647. doi: 10.1016/j.psj.2021.101647.

Shehata AM, Paswan VK, Attia YA, Abdel-Moneim AM, Abougabal MS, Sharaf M, et al., Managing gut microbiota through in ovo nutrition influences early-life programming in broiler chickens. Animals. 2021 Dec; 11(12): 3491. doi: 10.3390/ani11123491.

Araújo IC, Café MB, Noleto RA, Martins JM, Ulhoa CJ, Guareshi GC, et al., Effect of vitamin E in ovo feeding to broiler embryos on hatchability, chick quality, oxidative state, and performance. Poultry Science. 2019 Sep; 98(9): 3652-61. doi: 10.3382/ps/pey439.

Zakaria HA, Jalal M, Al-Titi HH, Souad A. Effect of sources and levels of dietary zinc on the performance, carcass traits and blood parameters of broilers. Brazilian Journal of Poultry Science. 2017 Jul; 19: 519-26. doi: 10.1590/1806-9061-2016-0415.

Bergoug H, Burel C, Guinebretiere M, Tong Q, Roulston N, Romanini CE, et al., Effect of pre-incubation and incubation conditions on hatchability, hatch time and hatch window, and effect of post-hatch handling on chick quality at placement. World's Poultry Science Journal. 2013 Jun; 69(2): 313-34. doi: 10.1017/S0043933913000329.

Araújo IC, Café MB, Noleto RA, Martins JM, Ulhoa CJ, Guareshi GC, et al., Effect of vitamin E in ovo feeding to broiler embryos on hatchability, chick quality, oxidative state, and performance. Poultry Science. 2019 Sep; 98(9): 3652-61. doi: 10.3382/ps/pey439.

Urso UR, Dahlke F, Maiorka A, Bueno IJ, Schneider AF, Surek D, et al., Vitamin E and selenium in broiler breeder diets: Effect on live performance, hatching process, and chick quality. Poultry Science. 2015 May; 94(5): 976-83. doi: 10.3382/ps/pev042.

Nnanle BP, Arge LW, Gbarasie VT, Suleiman IO, Ewuga EH, Igoche LA. Effect of storage period and position on physical egg quality characteristics and fertility of Guinea fowl eggs. Journal of Agriculture and Veterinary Science. 2017; 10(10): 68-72.

Al-Saeedi AH, Al-Fataftah AR, Al-Saleh AA, Al-Jaffar MA, Al-Smadi MA, Al-Azzam KM. Effect of in ovo injection with zinc methionine on growth performance, biochemical and histological parameters of broiler chickens. Animals. 2022; 12(2): 333.

Xu L, Li N, Farnell YZ, Wan X, Yang H, Zhong X, et al., Effect of feeding a high calcium: Phosphorus ratio, phosphorous deficient diet on hypophosphatemic rickets onset in broilers. Agriculture. 2021 Oct; 11(10): 955-67. doi: 10.3390/agriculture11100955.