Antibacterial Activity of Transition Metal Complexes of 2-(2-Hydroxybenzylidene) Hydrazinecarbothioamide: Antibacterial Activity of Transition Metal Complexes of Thiosemicarbazone Derivative

Hafiz Muhammad Ghuffran Qamar; Tehmina Bashir; Usman Ibrahim; Adnan Mehmood; Waiza Ansar; Noor Muhammad; Aygun Baghirova Alazova

doi:10.54393/fbt.v4i03.212

Authors

Hafiz Muhammad Ghuffran Qamar Department of Chemistry, Government College University, Lahore, Pakistan
Tehmina Bashir Department of Botany, Government Graduate College, Lahore, Pakistan
Usman Ibrahim Department of Chemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan
Adnan Mehmood Department of Microbiology, Gulab Devi Educational Complex, Lahore, Pakistan
Waiza Ansar Department of Zoology, Government College University, Lahore, Pakistan
Noor Muhammad Department of Zoology, Government College University, Lahore, Pakistan
Aygun Baghirova Alazova Institute of Botany, Ministry of Science and Education of the Republic of Azerbaijan, Azerbaijan

DOI:

https://doi.org/10.54393/fbt.v4i03.212

Keywords:

Thiosemicarbazone Complex, Metal Coordination, Antibacterial Activity, Bacillus licheniformis , Minimum Inhibitory Concentration, Minimum Bactericidal Concentration

Abstract

Thiosemicarbazone derivatives in the form of metal complexes have been sought after superior antibacterial effects than free ligands. Objectives: To prepare new Cu (II), Co (II), Mn (II), and Cd (II) complexes of 2 (2-hydroxybenzylidene) hydrazinecarbothioamide and assess their antimicrobial characteristics against some pathogenic microorganisms. Methods: This was a laboratory-based experimental study, which synthesized and characterized complexes by means of standard analytical and spectroscopic methods. Against Bacillus licheniformis, Escherichia coli, and Pseudomonas aeruginosa, the agar well diffusion method was evaluated at 12.5, 25, and 50 mg/mL. The values of MIC and MBC were calculated by the broth dilution method. The results were provided in the form of mean and SD, and statistical analysis was carried out by one-way ANOVA (p<0.05). Results: All the metal complexes showed concentration-dependent antibacterial activity. B. licheniformis was the most sensitive strain, in comparison to E. coli and P. aeruginosa. The Cu (II) and Mn (II) complexes had the strongest inhibitory effect, with the value of MIC and MBC, respectively, between 10 and 20mg/mL against B. licheniformis. Co (II) and Cd (II) complexes were moderately active depending on the strain of bacteria. Conclusions: Coordination of 2-(2-hydroxybenzylidene) hydrazinecarbothioamide with transition metals enhances antibacterial activity in a metal- and dose-dependent manner. Cu (II) and Mn (II) complexes displayed the most promising antibacterial properties, supporting the potential of metal-thiosemicarbazone complexes as candidates for developing new antimicrobial agents. Further mechanistic and toxicity studies are warranted.

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