Exploring Thermostable Lipases: Molecular Innovations and Expanding Industrial Horizons: Exploring Thermostable Lipases: Molecular Innovations

Tehmina Bashir; . Alamgir; Syeda Amna Batool; Tahir Hussain; . Washdev; Iram Rafique

doi:10.54393/fbt.v5i2.183

Authors

Tehmina Bashir Department of Botany, Government College University, Lahore, Pakistan
. Alamgir Faculty of Allied Health Sciences, Sohail University, Karachi, Pakistan
Syeda Amna Batool Department of Botany, University of Narowal, Narowal, Pakistan
Tahir Hussain Faculty of Allied Health Sciences, Hamdard Institute of Management Sciences, Karachi, Pakistan
. Washdev Chughtai Laboratory, Karachi, Pakistan
Iram Rafique Faculty of Allied Health Sciences, Karachi Institute of Nursing Allied Health Sciences, Karachi, Pakistan

DOI:

https://doi.org/10.54393/fbt.v5i2.183

Keywords:

Thermo-Stable Lipases, Commercial Uses, Characterization, Enzyme Engineering, Biocatalysts

Abstract

Thermostable lipases are crucial biocatalysts valued for their stability and functionality in both aqueous and non-aqueous environments, enabling efficient catalysis at elevated temperatures. These enzymes, derived from both wild-type and genetically engineered strains, exhibit unique properties that make them indispensable across diverse industrial sectors. Despite their potential, challenges remain in optimizing their production, purification, and characterization to meet specific application requirements ranging from highly purified pharmaceutical formulations to less refined industrial uses. This review consolidates recent advances in the isolation, engineering, and detailed characterization of thermostable lipases, highlighting their substrate specificity, catalytic efficiency, enantioselectivity, and tolerance to harsh conditions. Emphasis is placed on emerging molecular innovations and metagenomic approaches for discovering novel enzymes with enhanced industrial applicability. By bridging fundamental insights with practical applications, this overview aims to guide future research and development efforts in harnessing thermostable lipases for expanding biotechnological horizons.

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