Mixotrophic Cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production

Mixotrophic Cultivation of Chlorella vulgaris


  • Rabia Bano Department of Zoology, Division of Science, University of Education, Lahore, Pakistan
  • Azeem Azam Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Farhan Anjum Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Ata Ul Mustafa Fahid Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Hamza Faseeh Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Aqsa Riaz Department of Zoology, Wildlife & Fisheries, PMAS Arid Agriculture University, Rawalpindi, Pakistan




Bioenergy, Renewable Products, Chlorella vulgaris, Banana Peels, Molasses, Waste Utilization, Sustainable Cultivation


Environmentally friendly biofuels are currently produced in large quantities using algal lipids.  Objective: To perform mixotrophic cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production. Methods: Banana waste was treated with acid/alkaline, ground, and sun dried. The resultant hydrolysate was used into studies comparing photoautotrophic and mixotrophic conditions in microalgae culture. When biomass productivity and lipid content were measured. For mono-unsaturated, poly-unsaturated, and saturated forms, the lipid content differed. The research used analytical methods for fatty acid methyl ester analysis, such as GC-MS. Results: Mixotrophic cultivation exhibited a much higher biomass productivity (135 mg L-1 d-1) than photoautotrophic cultivation (115 mg L-1 d-1). Additionally, mixotrophically raised biomass had a much larger (w/w) lipid content (45%) than photo-autotrophically raised biomass (30 %). Higher amount of polyunsaturated fatty acids (palmitic and oleic acids) was shown by Lipidome. Conclusions: High-quality biofuel might be made possible by the regular availability of polyunsaturated fatty acids (64 mg g-1 of dry biomass) in the lipid contents of mixotrophically produced algal biomass


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DOI: 10.54393/fbt.v3i02.44
Published: 2023-09-30

How to Cite

Bano, R., Azam, A., Anjum, F., Fahid, A. U. M., Faseeh, H., & Riaz, A. (2023). Mixotrophic Cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production: Mixotrophic Cultivation of Chlorella vulgaris. Futuristic Biotechnology, 3(02), 41–45. https://doi.org/10.54393/fbt.v3i02.44