Mixotrophic Cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production

Mixotrophic Cultivation of Chlorella vulgaris


  • Rabia Bano Department of Zoology, Division of Science, University of Education, Lahore, Pakistan
  • Azeem Azam Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Farhan Anjum Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Ata Ul Mustafa Fahid Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Hamza Faseeh Institute of Zoology, University of the Punjab, Lahore, Pakistan
  • Aqsa Riaz Department of Zoology, Wildlife & Fisheries, PMAS Arid Agriculture University, Rawalpindi, Pakistan




Bioenergy, Renewable Products, Chlorella vulgaris, Banana Peels, Molasses, Waste Utilization, Sustainable Cultivation


Environmentally friendly biofuels are currently produced in large quantities using algal lipids.  Objective: To perform mixotrophic cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production. Methods: Banana waste was treated with acid/alkaline, ground, and sun dried. The resultant hydrolysate was used into studies comparing photoautotrophic and mixotrophic conditions in microalgae culture. When biomass productivity and lipid content were measured. For mono-unsaturated, poly-unsaturated, and saturated forms, the lipid content differed. The research used analytical methods for fatty acid methyl ester analysis, such as GC-MS. Results: Mixotrophic cultivation exhibited a much higher biomass productivity (135 mg L-1 d-1) than photoautotrophic cultivation (115 mg L-1 d-1). Additionally, mixotrophically raised biomass had a much larger (w/w) lipid content (45%) than photo-autotrophically raised biomass (30 %). Higher amount of polyunsaturated fatty acids (palmitic and oleic acids) was shown by Lipidome. Conclusions: High-quality biofuel might be made possible by the regular availability of polyunsaturated fatty acids (64 mg g-1 of dry biomass) in the lipid contents of mixotrophically produced algal biomass


Passos F, Gutiérrez R, Brockmann D, Steyer JP, García J, Ferrer I. Microalgae production in wastewater treatment systems, anaerobic digestion and modelling using ADM1. Algal Research. 2015 Jul; 10: 55-63. doi: 10.1016/j.algal.2015.04.008.

Gabina G, Martin L, Basurko OC, Clemente M, Aldekoa S, Uriondo Z. Performance of marine diesel engine in propulsion mode with a waste oil-based alternative fuel. Fuel. 2019 Jan; 235: 259-6. doi: 10.1016/j.fuel.2018.07.113.

Johnsson F, Kjärstad J, Rootzén J. The threat to climate change mitigation posed by the abundance of fossil fuels. Climate Policy. 2019 Feb; 19(2): 258-74. doi: 10.1080/14693062.2018.1483885.

Gautam K, Gupta NC, Sharma DK. Physical characterization and comparison of biodiesel produced from edible and non-edible oils of Madhucaindica (mahua), Pongamiapinnata (karanja), and Sesamumindicum (til) plant oilseeds. Biomass Conversion and Biorefinery. 2014 Sep; 4: 193-200. doi: 10.1007/s13399-013-0101-7.

Ende SS, and Noke A. Heterotrophic microalgae production on food waste and by-products. Journal of Applied Phycology. 2019 Jun; 31: 1565-71. doi: 10.1007/s10811-018-1697-6.

Kotrbáček V, Doubek J, Doucha J. The chlorococcalean alga Chlorella in animal nutrition: a review. Journal of Applied Phycology. 2015 Dec; 27: 2173-80. doi: 10.1007/s10811-014-0516-y.

Harun R, Danquah MK, Forde GM. Microalgal biomass as a fermentation feedstock for bioethanol production. Journal of Chemical Technology & Biotechnology. 2010 Feb; 85(2): 199-203. doi: 10.1002/jctb.2287.

Wijffels RH and Barbosa MJ. An outlook on microalgal biofuels. Science. 2010 Aug; 329(5993): 796-9. doi: 10.1126/science.1189003.

Malakar B, Das D, Mohanty K. Utilization of waste peel extract for cultivation of microalgal isolates: a study of lipid productivity and growth kinetics. Biomass Conversion and Biorefinery. 2022 Jan; 2022: 1-0. doi: 10.1007/s13399-022-02313-7.

Al-lwayzy SH, Yusaf T, Al-Juboori RA. Biofuels from the fresh water microalgae Chlorella vulgaris (FWM-CV) for diesel engines. Energies. 2014 Mar; 7(3): 1829-51 doi: 10.3390/en7031829.

Elahi MM, Hossain MM, Karim MR, Zain MF, Shearer C. A review on alkali-activated binders: Materials composition and fresh properties of concrete. Construction and Building Materials. 2020 Nov; 260: 119788. doi: 10.1016/j.conbuildmat.2020.119788.

Hossain MM, Karim MR, Elahi MM, Islam MN, Zain MF. Long-term durability properties of alkali-activated binders containing slag, fly ash, palm oil fuel ash and rice husk ash. Construction and Building Materials. 2020 Aug; 251: 119094. doi: 10.1016/j.conbuildmat.2020.119094.

Anwar Z, Gulfraz M, Irshad M. Agro-industrial lignocellulosic biomass a key to unlock the future bio-energy: a brief review. Journal of Radiation Research and Applied Sciences. 2014 Apr; 7(2): 163-73. doi: 0.1016/j.jrras.2014.02.003.

Shimizu FL, Monteiro PQ, Ghiraldi PH, Melati RB, Pagnocca FC, de Souza W, et al. Acid, alkali and peroxide pretreatments increase the cellulose accessibility and glucose yield of banana pseudostem. Industrial Crops and Products. 2018 May; 115: 62-8. doi: 10.1016/j.indcrop.2018.02.024.

Pelissari FM, Sobral PJ, Menegalli FC. Isolation and characterization of cellulose nanofibers from banana peels. Cellulose. 2014 Feb; 21: 417-32. doi: 10.1007/s10570-013-0138-6.

Arathi S, Kumar JT, Jothibasu K, Karthikeyan S, Suchitra R. Qualitative and quantitative estimation of algal lipids for biofuel production. International Journqal of Chemical Studies. 2020 Jun; 8: 2451-9. doi: 10.22271/chemi.2020.v8.i4ab.10003.

Karim R, Nahar K, Zohora FT, Islam MM, Bhuiyan RH, Jahan MS, et al. Pectin from lemon and mango peel: Extraction, characterisation and application in biodegradable film. Carbohydrate Polymer Technologies and Applications. 2022 Dec; 4: 100258. doi: 10.1016/j.carpta.2022.100258.

Manzoor A, Ahmad S, Yousuf B. Effect of bioactive-rich mango peel extract on physicochemical, antioxidant and functional characteristics of chicken sausage. Applied Food Research. 2022 Dec; 2(2): 100183. doi: 10.1016/j.afres.2022.100183.

Chaudhry WN, Jamil N, Ali I, Ayaz MH, Hasnain S. Screening for polyhydroxyalkanoate (PHA)-producing bacterial strains and comparison of PHA production from various inexpensive carbon sources. Annals of Microbiology. 2011 Sep; 61(3): 623-9. doi: 10.1007/s13213-010-0181-6.

Fan J, Huang J, Li Y, Han F, Wang J, Li X, et al. Sequential heterotrophy–dilution–photo induction cultivation for efficient microalgal biomass and lipid production. Bioresource Technology. 2012 May; 112: 206-11. doi: 10.1016/j.biortech.2012.02.046.



DOI: 10.54393/fbt.v3i02.44
Published: 2023-09-30

How to Cite

Bano, R., Azam, A., Anjum, F., Fahid, A. U. M., Faseeh, H., & Riaz, A. (2023). Mixotrophic Cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production: Mixotrophic Cultivation of Chlorella vulgaris. Futuristic Biotechnology, 3(02), 41–45. https://doi.org/10.54393/fbt.v3i02.44


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