Mixotrophic Cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production: Mixotrophic Cultivation of Chlorella vulgaris

Rabia Bano; Azeem Azam; Farhan Anjum; Ata Ul Mustafa Fahid; Hamza Faseeh; Aqsa Riaz

doi:10.54393/fbt.v3i02.44

Authors

Rabia Bano Department of Zoology, Division of Science, University of Education, Lahore, Pakistan
Azeem Azam Institute of Zoology, University of the Punjab, Lahore, Pakistan
Farhan Anjum Institute of Zoology, University of the Punjab, Lahore, Pakistan
Ata Ul Mustafa Fahid Institute of Zoology, University of the Punjab, Lahore, Pakistan
Hamza Faseeh Institute of Zoology, University of the Punjab, Lahore, Pakistan
Aqsa Riaz Department of Zoology, Wildlife & Fisheries, PMAS Arid Agriculture University, Rawalpindi, Pakistan

DOI:

https://doi.org/10.54393/fbt.v3i02.44

Keywords:

Bioenergy, Renewable Products, Chlorella vulgaris, Banana Peels, Molasses, Waste Utilization, Sustainable Cultivation

Abstract

Environmentally friendly biofuels are currently produced in large quantities using algal lipids. Objective: To perform mixotrophic cultivation of Chlorella vulgaris on Banana Waste for Biodiesel Production. Methods: Banana waste was treated with acid/alkaline, ground, and sun dried. The resultant hydrolysate was used into studies comparing photoautotrophic and mixotrophic conditions in microalgae culture. When biomass productivity and lipid content were measured. For mono-unsaturated, poly-unsaturated, and saturated forms, the lipid content differed. The research used analytical methods for fatty acid methyl ester analysis, such as GC-MS. Results: Mixotrophic cultivation exhibited a much higher biomass productivity (135 mg L-1 d-1) than photoautotrophic cultivation (115 mg L-1 d-1). Additionally, mixotrophically raised biomass had a much larger (w/w) lipid content (45%) than photo-autotrophically raised biomass (30 %). Higher amount of polyunsaturated fatty acids (palmitic and oleic acids) was shown by Lipidome. Conclusions: High-quality biofuel might be made possible by the regular availability of polyunsaturated fatty acids (64 mg g-1 of dry biomass) in the lipid contents of mixotrophically produced algal biomass

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