Green Synthesis of Copper Nanoparticles from Artemisia Maritima: Characterization and Evaluation of Antibacterial Properties  : Green Synthesis of Cu NPs from Artemisia Maritima: Antibacterial Properties

Saad Abbasi; Hammad Ahmed Abbasi; Muhammad Atif; Muhammad Naveed Anjum; Ubaid Ur Rehman

doi:10.54393/fbt.v4i04.168

Authors

Saad Abbasi Department of Biotechnology, International Islamic University, Islamabad, Pakistan
Hammad Ahmed Abbasi Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Muhammad Atif Center of Applied Molecular Biology, University of the Punjab, Lahore, Pakistan
Muhammad Naveed Anjum Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Ubaid Ur Rehman Center of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan

DOI:

https://doi.org/10.54393/fbt.v4i04.168

Keywords:

Copper Nanoparticles, Artemisia Maritima, Aqueous Extract, Antibacterial Properties

Abstract

Copper nanoparticles (Cu NPs) attracted many researchers due to their potential biomedical and pharmacological activities that depend on the shape and size of the nanoparticles. Objective: To extract and characterize nanoparticles from the aqueous extract of the Artemisia maritima plant. Methods: UV-V spectroscopy indicates the presence of Cu NPs with unique optical characteristics. FTIR analysis identified functional groups and chemical bonds in the Cu NPs. XRD analysis revealed a hexagonal crystal structure for the Cu NPs. Antibacterial activity of the synthesized Cu NPs was evaluated against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus. Results: The Cu NPs exhibited varying zones of inhibition (ZOI) against different bacterial strains, with the largest ZOI observed against Staphylococcus aureus (20 mm), followed by Bacillus subtilis (19 mm), Pseudomonas aeruginosa (17 mm), Klebsiella pneumoniae (16 mm), and Escherichia coli (11 mm). These results highlight the potential of Artemisia maritima-synthesized Cu NPs as antimicrobial agents, particularly against Gram-positive bacteria. Conclusions: Artemisia maritima-mediated Cu NPs offer a promising, green alternative for antimicrobial development, warranting further research for clinical and environmental applications.

References

Adewumi OA, Singh V, Singh G. Chemical Composition, Traditional Uses and Biological Activities of Artemisia Species. Journal of Pharmacognosy and Phytochemistry. 2020; 9(5): 1124-40.

Hameed A, Zafar M, Ullah R, Shahat AA, Ahmad M, Cheema SI et al. Systematic Significance of Pollen Morphology and Foliar Epidermal Anatomy of Medicinal Plants Using SEM and LM Techniques. Microscopy Research and Technique. 2020 Aug; 83(8): 1007-22.

Siwan D, Nandave D, Nandave M. Artemisia Vulgaris Linn: An Updated Review on Its Multiple Biological Activities. Future Journal of Pharmaceutical Sciences. 2022 Nov; 8(1): 47. doi: 10.1186/s43094-022-00436-2.

Hasan HA. Tarragon (Artemisia dracunculus L.) Biological Medicinal, Nutritional and Economic Plant. Journal of Kirkuk University for Agricultural Sciences. 2024 Sep; 15(3).

Chae GC, Auh Q, Chun YH, Hong JP. Antibacterial Activity of Artemisia Capillaris THUNB on Oral Bacteria. Journal of Oral Medicine and Pain. 2009; 34(2): 169-77.

Hong SH, Seo SH, Lee JH, Choi BT. The Aqueous Extract from Artemisia Capillaris Thunb. Inhibits Lipopolysaccharide-Induced Inflammatory Response Through Preventing NF-Κb Activation in Human Hepatoma Cell Line and Rat Liver. International Journal of Molecular Medicine. 2004 May; 13(5): 717-20. doi: 10.3892/ijmm.13.5.717.

Davis BA, Fasel M, Kaplan JO, Russo E, Burke A. The climate and vegetation of Europe, North Africa and the Middle East during the Last Glacial Maximum (21,000 years BP) based on pollen data. Climate of the Past Discussions. 2022 Aug; 2022: 1-66. doi: 10.5194/cp-2022-59.

Shah SM, Nisar Z, Nisar J, Akram M, Ghotekar S, Oza R. Nano-biomedicine: A New Approach of Medicinal Plants and Their Therapeutic Modalities. Journal of Materials and Environmental Science. 2021; 12: 1-4.

Rasheed T, Bilal M, Iqbal HM, Li C. Green Biosynthesis of Silver Nanoparticles Using Leaves Extract of Artemisia Vulgaris and Their Potential Biomedical Applications. Colloids and Surfaces B: Bio-interfaces. 2017 Oct; 158: 408-15. doi: 10.1016/j.colsurfb.2017.07.020.

Hu C, Li HX, Zhang MT, Liu LF. Structure Characterization and Anticoagulant Activity of a Novel Polysaccharide from Leonurus Artemisia (Laur.) SY Hu F. RSC Advances. 2020; 10(4): 2254-66. doi: 10.1039/C9RA10853J.

Roy K, Srivastwa AK, Ghosh CK. Anticoagulant, Thrombolytic and Antibacterial Activities of Euphorbia Acruensis Latex-Mediated Bioengineered Silver Nanoparticles. Green Processing and Synthesis. 2019 Jan 28;8(1):590-9. doi: 10.1515/gps-2019-0029.

Shahriary S, Tafvizi F, Khodarahmi P, Shaabanzadeh M. Phyto-mediated synthesis of CuO Nanoparticles Using Aqueous Leaf Extract of Artemisia Deserti and Their Anticancer Effects on A2780-CP Cisplatin-Resistant Ovarian Cancer Cells. Biomass Conversion and Bio-refinery. 2024 Jan; 14(2): 2263-79. doi: 10.1007/s13399-022-02436-x.

Taghavizadeh Yazdi ME, Darroudi M, Amiri MS, Hosseini HA, Nourbakhsh F, Mashreghi M et al. Anticancer, Antimicrobial, and Dye Degradation Activity of Biosynthesised Silver Nanoparticle Using Artemisia Kopetdaghensis. Micro and Nano Letters. 2020 Dec; 15(14): 1046-50. doi: 10.1049/mnl.2020.0387.

Aslany S, Tafvizi F, Naseh V. Characterization and Evaluation of Cytotoxic and Apoptotic Effects of Green Synthesis of Silver Nanoparticles Using Artemisia Ciniformis on Human Gastric Adenocarcinoma. Materials Today Communications. 2020 Sep; 24: 101011. doi: 10.1016/j.mtcomm.2020.101011.

Mousavi B, Tafvizi F, Zaker Bostanabad S. Green Synthesis of Silver Nanoparticles Using Artemisia Turcomanica Leaf Extract and the Study of Anti-Cancer Effect and Apoptosis Induction on Gastric Cancer Cell Line (AGS). Artificial Cells, Nano-medicine, and Biotechnology. 2018 Oct; 46(sup1): 499-510. doi: 10.1080/21691401.2018.1430697.

Avitabile E, Senes N, D’avino C, Tsamesidis I, Pinna A, Medici S et al. The Potential Antimalarial Efficacy of Hemocompatible Silver Nanoparticles from Artemisia Species Against P. Falciparum Parasite. PLOS ONE. 2020 Sep; 15(9): e0238532. doi: 10.1371/journal.pone.0238532.

Akbari M, Morad R, Maaza M. First Principle Study of Silver Nanoparticle Interactions with Antimalarial Drugs Extracted from Artemisia Annua Plant. Journal of Nanoparticle Research. 2020 Nov; 22(11): 331. doi: 10.1007/s11051-020-05058-4.

Soltys L, Olkhovyy O, Tatarchuk T, Naushad M. Green Synthesis of Metal and Metal Oxide Nanoparticles: Principles of Green Chemistry and Raw Materials. Magneto-chemistry. 2021 Oct; 7(11): 145. doi: 10.3390/magnetochemistry7110145.

Sathiyaraj S, Suriyakala G, Gandhi AD, Babujanarthanam R, Almaary KS, Chen TW, Kaviyarasu K. Biosynthesis, Characterization, and Antibacterial Activity of Gold Nanoparticles. Journal of Infection and Public Health. 2021 Dec; 14(12): 1842-7. doi: 10.1016/j.jiph.2021.10.007.

Puthukulangara Jaison J, Kadanthottu Sebastian J. Photocatalytic and Antioxidant Potential of Silver Nanoparticles Biosynthesized Using Artemisia Stelleriana Leaf Extracts. Water Practice and Technology. 2023 Nov; 18(11): 2664-74.doi: 10.2166/wpt.2023.176.

Woźniak-Budych MJ, Staszak K, Staszak M. Copper and Copper-Based Nanoparticles in Medicine—Perspectives and Challenges. Molecules. 2023 Sep; 28(18): 6687. doi: 10.3390/molecules28186687.

Lai MJ, Huang YW, Chen HC, Tsao LI, Chang Chien CF, Singh B et al. Effect of Size and Concentration of Copper Nanoparticles on the Antimicrobial Activity in Escherichia Coli Through Multiple Mechanisms. Nanomaterials. 2022 Oct; 12(21): 3715. doi: 10.3390/nano12213715.