Detection of Tuberculosis on Culture, Comparison of Findings with Fluorescence Microscopy and GeneXpert: Comparison of GeneXpert, Fluorescence Microscopy with Culture

Asmat Amjad; Zeeshan Yousuf; Hafiz Shehzad Muzammil

doi:10.54393/fbt.v1i01.6

Authors

Asmat Amjad Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
Zeeshan Yousuf The Islamia University Bahawalpur, Bahawalpur, Pakistan
Hafiz Shehzad Muzammil National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan

DOI:

https://doi.org/10.54393/fbt.v1i01.6

Keywords:

Tuberculosis, Culture, Fluorescence Microscope, Sensitivity, GeneXpert

Abstract

The GeneXpert MTB/RIF technique is used for the identification of tuberculosis and rifampicin (RIF) resistance. Xpert MTB / RIF provides patients with distinct advantages such as early diagnosis. Objectives: To compared the performance of fluorescence microscopy and GeneXpert with culture in TB samples from Narowal, Pakistan. Methods: A total of 299 TB positive specimens were obtained. Among these 54% (n = 160) were categorized to be obtained from male and 46% (n = 139) from female population. The sensitivity and specificity of fluorescence microscopy, GeneXpert and culture of TB samples were done. Results: The parameters including sensitivity and specificity calculated for GeneXpert were 73% and 100%, respectively, while the sensitivity and specificity calculated for culture was 100% and the sensitivity and specificity for FM microscope were 43% and 100%, respectively. Conclusions: We conclude that the GeneXpert is more sensitive than FM considering culture as a gold standard. Although the GeneXpert assay was also shown to be able to detect a limited number of bacillus from samples, the culture's sensitivity and specificity were both 100%.

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