Evaluating the Composition of Biodiesel Synthesized from Black Soldier Fly (Hermetia illucens) Larvae: Biodiesel from Black Soldier Fly Larvae

Fatima Khizar; Sana Hameed; Hafiz Kamran Yousaf; Muhammad Sajjad Sarwar

doi:10.54393/fbt.v4i02.125

Authors

Fatima Khizar Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
Sana Hameed Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
Hafiz Kamran Yousaf Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan
Muhammad Sajjad Sarwar Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan

DOI:

https://doi.org/10.54393/fbt.v4i02.125

Keywords:

Biodiesel, Black Soldier Fly Larvae, Transesterification, Sustainable, Feedstock

Abstract

Biodiesel is considered a viable alternative to conventional diesel, particularly for the ground transportation industry. While different plant seeds oils have been the dominant feedstocks for biodiesel synthesis to date. However, they are often expensive due to their limited supply and low reproductive rate. Objective: To present a sustainable approach by using the black soldier fly (Hermetia illucens) larvae as an alternative feedstock. Methods: The larvae were fed with waste chicken rice, fish, soft vegetables and fruits. These wastes provide food and shelter for disease-causing insect larvae and contributes to land pollution. Using petroleum ether as a solvent, 12.2 g of crude grease was extracted from ~ 500 larvae, resulting in about 11.8 g of biodiesel through a two-step acid-base catalyzed transesterification process. Results: The resultant biodiesel was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), revealing a Fatty Acid Methyl Ester (FAME) profile predominantly comprising dodecanoic acid, 9-Hexadecenoic acid, 9- octadecenoic acid and 11- octadecenoic acid etc, emphasizing its potential as a high-quality alternative to conventional diesel fuel. Conclusions: This study contributed to develop biodiesel as an eco-friendly renewable energy technology by using BSFL.

References

Kirsch S. Running out? Rethinking resource depletion. The extractive industries and society. 2020 Jul; 7(3): 838-40. doi: 10.1016/j.exis.2020.06.002.

Martins F, Felgueiras C, Smitkova M, Caetano N. Analysis of fossil fuel energy consumption and environmental impacts in European countries. Energies. 2019 Mar; 12(6): 964. doi: 10.3390/en12060964.

Eswaran N, Parameswaran S, Johnson TS. Biofuels and sustainability. Biofuels and Biodiesel. 2021 May: 317-42. doi: 10.1007/978-1-0716-1323-8_20.

Ogunkunle O and Ahmed NA. A review of global current scenario of biodiesel adoption and combustion in vehicular diesel engines. Energy Reports. 2019 Nov; 5: 1560-79. doi: 10.1016/j.egyr.2019.10.028.

Mamat R, Sani MS, Sudhakar K, Kadarohman A, Sardjono RE. An overview of higher alcohol and biodiesel as alternative fuels in engines. Energy Reports. 2019 Nov; 5: 467-79. doi: 10.1016/j.egyr.2019.04.009.

Bórawski P, Bełdycka-Bórawska A, Szymańska EJ, Jankowski KJ, Dubis B, Dunn JW. Development of renewable energy sources market and biofuels in The European Union. Journal of Cleaner Production. 2019 Aug; 228: 467-84. doi: 10.1016/j.jclepro.2019.04.242.

Singh D, Sharma D, Soni SL, Sharma S, Kumari D. Chemical compositions, properties, and standards for different generation biodiesels: A Review Fuel. 2019 Oct; 253: 60-71. doi: 10.1016/j.fuel.2019.04.174.

Chozhavendhan S, Singh MV, Fransila B, Kumar RP, Devi GK. A review on influencing parameters of biodiesel production and purification processes. Current Research in Green and Sustainable Chemistry. 2020 Feb; 1: 1-6. doi: 10.1016/j.crgsc.2020.04.002.

Animasaun DA, Ameen MO, Belewu MA. Protocol for Biodiesel Production by Base-Catalyzed Transesterification Method. InBiofuels and Biodiesel. 2021 May; 103-113. doi: 10.1007/978-1-0716-1323-8_7.

Chen L, Debnath D, Zhong J, Ferin K, VanLoocke A, Khanna M. The economic and environmental costs and benefits of the renewable fuel standard. Environmental Research Letters. 2021 Feb; 16(3): 034021. doi: 10.1088/1748-9326/abd7af.

Ewusie EA, Kwapong PK, Ofosu-Budu G, Sandrock C, Akumah AM, Nartey EK et al. The black soldier fly, Hermetia illucens (Diptera: Stratiomyidae): Trapping and culturing of wild colonies in Ghana. Scientific African. 2019 Sep; 5: e00134. doi: 10.1016/j.sciaf.2019.e00134.

Barragan‐Fonseca KB, Dicke M, van Loon JJ. Influence of larval density and dietary nutrient concentration on performance, body protein, and fat contents of black soldier fly larvae (Hermetia illucens). Entomologia Experimentalis et Applicata. 2018 Sep; 166(9): 761-70. doi: 10.1111/eea.12716.

Kim CH, Ryu J, Lee J, Ko K, Lee JY, Park KY et al. Use of black soldier fly larvae for food waste treatment and energy production in Asian countries: a review. Processes. 2021 Jan; 9(1): 161. doi: 10.3390/pr9010161.

Nguyen HC, Liang SH, Li SY, Su CH, Chien CC, Chen YJ et al. Direct transesterification of black soldier fly larvae (Hermetia illucens) for biodiesel production. Journal of the Taiwan Institute of Chemical Engineers. 2018 Apr; 85: 165-9. doi: 10.1016/j.jtice.2018.01.035.

Canakci M and Sanli H. Biodiesel production from various feedstocks and their effects on the fuel properties. Journal of Industrial Microbiology and Biotechnology. 2008 May; 35(5): 431-41. doi: 10.1007/s10295-008-0337-6.

Park HH. Black soldier fly larvae manual. 2016.

Singh A and Kumari K. An inclusive approach for organic waste treatment and valorisation using Black Soldier Fly larvae: A review. Journal of Environmental Management. 2019 Dec; 251: 109569. doi: 10.1016/j.jenvman.2019.109569.

Wang YS and Shelomi M. Review of black soldier fly (Hermetia illucens) as animal feed and human food. Foods. 2017 Oct; 6(10): 91. doi: 10.3390/foods6100091.

Pauline JM, Sivaramakrishnan R, Pugazhendhi A, Anbarasan T, Achary A. Transesterification kinetics of waste cooking oil and its diesel engine performance. Fuel. 2021 Feb; 285: 119108. doi: 10.1016/j.fuel.2020.119108.

Lim JW, Mohd-Noor SN, Wong CY, Lam MK, Goh PS, Beniers JJ et al. Palatability of black soldier fly larvae in valorizing mixed waste coconut endosperm and soybean curd residue into larval lipid and protein sources. Journal of Environmental Management. 2019 Feb; 231: 129-36. doi: 10.1016/j.jenvman.2018.10.022.

Poudyal R, Loskot P, Nepal R, Parajuli R, Khadka SK. Mitigating the current energy crisis in Nepal with renewable energy sources. Renewable and Sustainable Energy Reviews. 2019 Dec; 116: 109388. doi: 10.1016/j.rser.2019.109388.

Sharma B and Shrestha A. Petroleum dependence in developing countries with an emphasis on Nepal and potential keys. Energy Strategy Reviews. 2023 Jan; 45: 101053. doi: 10.1016/j.esr.2023.101053.

Zheng L, Li Q, Zhang J, Yu Z. Double the biodiesel yield: Rearing black soldier fly larvae, Hermetia illucens, on solid residual fraction of restaurant waste after grease extraction for biodiesel production. Renewable Energy. 2012 May; 41: 75-9. doi: 10.1016/j.renene.2011.10.004.

Park JY, Jung S, Na YG, Jeon CH, Cheon HY, Yun EY et al. Biodiesel production from the black soldier fly larvae grown on food waste and its fuel property characterization as a potential transportation fuel. Environmental Engineering Research. 2022 Jun; 27(3). doi: 10.4491/eer.2020.704.

Srisuksai K, Limudomporn P, Kovitvadhi U, Thongsuwan K, Imaram W, Lertchaiyongphanit R et al. Physicochemical properties and fatty acid profile of oil extracted from black soldier fly larvae (Hermetia illucens). Veterinary World. 2024 Mar; 17(3): 518. doi: 10.14202/vetworld.2024.518-526.

Addeo NF, Li C, Rusch TW, Dickerson AJ, Tarone AM, Bovera F et al. Impact of age, size, and sex on adult black soldier fly [Hermetia illucens L.(Diptera: Stratiomyidae)] thermal preference. Journal of Insects as Food and Feed. 2022 Feb; 8(2): 129-39. doi: 10.3920/JIFF2021.0076.